DNA-templated Ag nanocluster formation.

TitleDNA-templated Ag nanocluster formation.
Publication TypeJournal Article
Year of Publication2004
AuthorsPetty, JT, Zheng, J, Hud, NV, Dickson, RM
JournalJ Am Chem Soc
Volume126
Issue16
Pagination5207-12
Date Published2004 Apr 28
ISSN0002-7863
KeywordsCircular Dichroism, DNA, Kinetics, Magnetic Resonance Spectroscopy, Nucleic Acid Conformation, Oligonucleotides, Silver, Spectrometry, Fluorescence, Spectrometry, Mass, Electrospray Ionization, Templates, Genetic
Abstract

The high affinity of Ag+ for DNA bases has enabled creation of short oligonucleotide-encapsulated Ag nanoclusters without formation of large nanoparticles. Time-dependent formation of cluster sizes ranging from Ag1 to Ag4/oligonucleotide were observed with strong, characteristic electronic transitions between 400 and 600 nm. The slow nanocluster formation kinetics enables observation of specific aqueous nanocluster absorptions that evolve over a period of 12 h. Induced circular dichroism bands confirm that the nanoclusters are associated with the chiral ss-DNA template. Fluorescence, absorption, mass, and NMR spectra all indicate that multiple species are present, but that their creation is both nucleotide- and time-dependent.

DOI10.1021/ja031931o
Alternate JournalJ. Am. Chem. Soc.
PubMed ID15099104
Grant List1R01GM068731 / GM / NIGMS NIH HHS / United States