Christina Y. Hampton
Office: ES&T L2-110
Office Phone: 404-385-4427
2004 - Present Georgia Institute of Technology, Ph.D.
Student, Analytical Chemistry
2003 Western Washington University, B.S. Chemistry
2001 Pierce Community College, A.A.S.
Honors and Awards
Molecular Biophysics Trainee, Georgia Institute of Technology
Pfizer Graduate Travel Award
Molecular Biophysics Trainee, Georgia Institute of Technology
Richard A. Schaeffer ASMS Travel Award Recipient, Extrel
CETL/BP Outstanding Teaching Assistant Nominee, Georgia Institute of
Outstanding Teaching Assistant in the School of Chemistry &
Biochemistry, Georgia Institute of Technology
GAANN Fellowship, Georgia Institute of Technology
Harborstone Credit Union Scholarship, Western Washington University
Outstanding Math Student, Pierce Community College
Micromachined Ultrasonic Electrospray (AMUSE) Ion Sources
The increasing use of mass
spectrometry (MS) in proteomics and metabonomics has generated an
urgent need for the development of higher throughput ionization
sources which are not subject to the intrinsic limitations of
conventional electrospray ionization (ESI), namely, the requirement
for high voltages (1-5 kV) to produce liquid atomization, limited
dynamic range due to charge transport restrictions and the need to
use organic solvent to improve gaseous ion formation. We have shown
that the Array of Micromachined UltraSonic Electrosprays (AMUSE),
invented and developed by Fedorov and Degertekin , and
demonstrated on a mass spectrometry system jointly with our group
, has the potential of overcoming these limitations. The AMUSE
decouples the processes of droplet formation and droplet charging by
acoustically forcing the ejection of micron-sized droplets from an
array with 400 1-5 micron
diameter nozzles using an RF potential, while applying a low voltage
(100 V) DC potential directly to an aqueous analyte solution. A
Venturi device incorporated into the setup increases the linear
velocity of the droplet flow causing a corresponding increase in the
ion transfer efficiency. Thus far, we have demonstrated the
effectiveness of the AMUSE for ionizing standard compounds prepared
in acidified aqueous solvent mixtures, even in the absence of DC
potentials and our most recent experiments to measure the internal
energy deposition of this source using thermometer compounds suggest
that ionization by the AMUSE may be softer than that of conventional
ESI, producing ions with more controllable salvation shells,
supporting our hypothesis that the AMUSE could find widespread
applicability in proteomics and suggesting that it may be useful in
studies designed to probe protein conformation and macromolecular
 Fedorov, A. G.
and Degertekin, F. L., "Electrospray Systems and Methods", U.S.
Patent 7,208,727 (04/24/2007) and U.S. Patent App. 11/594,489
 Aderogba, S.,
et al., Appl. Phys. Lett., 86, 203110-203113 (2005).
AMUSE Ejection Movie (3.8 MB)
This movie shows ejection by
Movie (4.3 MB) This movie shows the increase in the linear
velocity of the ejected droplets produced by the AMUSE as a result
of coupling a Venturi device to the AMUSE.
Direct Analysis in Real Time
(DART) Screening of Drugs
In many areas of SE Asia, small
combination packs of 4-5 drugs ('yaa chud' in Thai) are sold in
shops and pharmacies for the treatment of malaria and its associated
health effects. These yaa chud are sold without prescription or
medical assessment and may contain pharmaceuticals that can
effectively treat malaria but more often contain drugs that treat
malarial symptoms. This practice poses a serious health threat
as these non-efficacious drugs will not improve the long-term health
of the patient, may be contraindicated in pregnancy or childhood,
and may increase the spread of drug-resistant malaria. DART is a
high-throughput ionization source was used to screen a set of 50 yaa
chud bags (182 pills) purchased along the Thai-Burma Border. Only
14% of these yaa chud contained medicine that could have cured
malaria while 82% contained medicines that were contraindicated in
Click here to see the results of this
survey Yaa Chud
Metabolism of Calpain Inhibitors
Many acute and chronic neurological
disorders such as stroke, spinal cord and head injuries and multiple
sclerosis are characterized by severe axonal degeneration. Calpain
inhibitors are a class of drugs that prevent axonal degeneration by
reducing the level of intracellular calcium and deactivating calpain
proteases preventing axonal destruction. We have developed a
LC/MS method to quantify the levels of these drugs and their
metabolites in serum and tissue samples in an effort to determine
the appropriate therapeutic dose as well as to track the
distribution of these drugs throughout the body. We are also
developing an atmospheric-pressure MALDI method to increase the
throughput of these analyses as the limiting factor the LC/MS method
is the long time that it takes to prepare and analyze the samples.
"Comparison of the Internal Energy Deposition of Direct Analysis in
Real Time and Electrospray Ionization Time-of-Flight Mass
Spectrometry Ionization Techniques", Glenn A. Harris, D. M.
Hostetler, Christina. Y. Hampton, F. M. Fernandez, 2009, Accepted.
"A Comprehensive Comparison of Support Vector Machine Classifiers of
Ovarian Cancer Metabolomic Data", W. Guan, M. Zhou, Christina Y.
Hampton, A. Gray, J. F. McDonald, F. M. Fernandez, BMC
Bioinformatics, 2009, In Preparation.
random survey of the proportion of poor quality oral artesunate sold
at medicine outlets in the Lao PDR - implications for therapeutic
failure and drug resistance", Sivong Sengaloundeth, Michael D.
Green, Facundo M. Fernandez, Ot Manolin, Khamlieng Phommavong,
Vongsavanh Insixiengmay, Christina Y. Hampton, Leonard Nyadong,
Dallas Mildenhall, Lamphet Khounsaknalath, Lamphone Syhakhang, Paul
N. Newton, accepted in Malaria J.
"Liquid Chromatography and Ambient Ionization Time-of-flight Mass
Spectrometry for the Analysis of Genuine and Counterfeit
Pharmaceuticals", Facundo M. Fernández, Christina Y. Hampton,
Leonard Nyadong, Arti Navare, Mark Kwasnik in LC/TOF-MS for
Accurate Mass Analysis: Principles and Applications, ed. by I.
Ferrer and E.M. Thurman, Wiley: 2009.
"Characterisation of 'Yaa Chud' Medicine on the Thailand-Myanmar
Border: Selecting for Drug-resistant Malaria and Threatening Public
Health", Paul N. Newton, Christina Y. Hampton, Krystyn
Alter-Hall, Thanongsak Teerwarakulpana, Sompol Prakongpan, Ronnatrai
Ruangveerayuth, Nicholas J. White, Nicholas P. J. Day, Mabel B.
Tudino, Natalia Mancuso, Facundo M. Fernández, Am. J. Trop. Med.
Hyg., 2008, 79, 662-669.
"Comparison of Internal Energy
Deposition of Venturi-assisted Ionization Sources", Christina Y.
Hampton, Catherine J. Silvestri, Thomas P. Forbes, Mark J.
Varady, J. Mark Meacham, Andrei G. Fedorov, F. Levent Degertekin,
Facundo M. Fernández, J. Am. Soc. Mass Spectrom.,
epidemiological investigation into the criminal fake artesunate
trade: an international collaboration between police, scientists,
and health workers", Paul N Newton, Facundo M. Fernández, Aline
Plancon-Lecadre, Dallas Mildenhall, Michael D Green, Li Ziyong, Eva
Maria Christophel, Souly Phanouvong, Stephen Howells, Eric MacIntosh,
Paul Laurin, Nancy Blum, Christina Y. Hampton, Kevin Faure, Leonard
Nyadong, C.W.R. Soong, Budiono Santoso, Wang Zhiguang, John Newton,
Kevin Palmer, PLoS Med., 2007, 5(2), e32.
Performance of a Venturi-assisted Array of Micromachined UltraSonic
Electrosprays (AMUSE) Coupled to Ion Trap Mass Spectrometry for the
Analysis of Peptides and Proteins", Christina Y. Hampton, Thomas P.
Forbes, Mark J. Varady, J. Mark Meacham, Andrei G. Fedorov, F.
Levent Degertekin, Facundo M. Fernández, Anal. Chem.,
"Characterization of Solid Counterfeit Drug Samples by Desorption
Electrospray Ionization and Direct-analysis-in-real-time Coupled to
Time-of-flight Mass Spectrometry". Facundo M. Fernández,
Robert B. Cody, Michael D. Green, Christina Y. Hampton, Rose McGready, Sivong Sengaloundeth, Nicholas J. White, Paul N. Newton.
ChemMedChem, 2006, 1, 702-705.