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DESI Set-up

 

Asiri S. Galhena                                                                                           

Office:  ES&T L2-114

Office Phone:  404-385-4427

E-mail: agalhena3@mail.gatech.edu

 

Education                                                                                              

June 2008 - Present     Georgia Institute of Technology, Post-Doc

2003 - 2008                University of Arizona, Ph.D. Analytical Chemistry         

1998 - 2002                University of Colombo, Sri Lanka, B.S. Chemistry

 

Research Interests

Imaging Desorption Electrospray Ionization Mass Spectrometry (MS)

 

        Desorption electrospray ionization (DESI) is a novel ionization techniques that permits the analysis of

        surfaces without a sample preparation or a treatment step while the sample is being held under

        ambient conditions. DESI is a desorption ionization (DI) method in which the surface being

        analyzed is impacted by charged droplets of solvent generated from an electrospray. One potential

        application of DESI is for the analysis of analytes distributed about a surface. In other words, DESI

        can be used to construct chemical images off of surfaces. Current research involves the

        develpment and improvement of DESI-MS imaging technique for direct analysis of biological and

        pharmaceutical samples.

 

Publications

  1. "Enhanced Direct Ambient Analysis By Differenctial Mobility-Filtered Desorption Electrospray Ionization-Mass Spectrometry", Asiri S. Galhena, Glenn A. Harris, Mark Kwasnik, Facundo M. Fernandez, Analytical Chemistry, 2010, 82(22), 9159-9163.

  3. "Small Molecule Ambient Mass Spectrometry Imaging by Infrared Laser Ablation Metastable-Induced Chemical Ionization", A. S. Galhena, Glenn A. Harris, Kermit K. Murray, Facundo M. Fernandez, Analytical Chemistry, 2010, 82(6), 2178-2181.

  4. "Microplasma Discharge Ionization Source for Ambient Mass Spectrometry", Joshua Symonds, A. S. Galhena, Facundo M. Fernandez, Thomas Orlando, Anal. Chem., 2010, 82, 621-627.

  5. "Beta Electron-Assisted Direct Chemical Ionization (BADCI) Probe for Ambient Mass Spectrometry", J. Steeb, A. S. Galhena, L. Nyadong, J. Janata, F. M. Fernandez. Chem. Commun. 2009, In Press.

  6. "Desorption Electrospray/Metastable-Induced Ionization (DEMI): A Flexible Multimode Ambient Ion Generation Technique", L. Nyadong, A. S. Galhena, F. M. Fernandez. Anal. Chem. 2009, Accepted.

  7. "Combining Two-Dimensional Diffusion-Ordered Nuclear Magnetic Resonance Spectroscopy, Imaging Desorption Electrospray Ionization Mass Spectrometry and Direct Analysis in Real Time Mass Spectrometry for the Integral Investigation of Counterfeit Pharmaceuticals", L. Nyadong*, G. A. Harris*, S. Balayssac*, A. S. Galhena*, M. Malet-Martino, R. Martino, R. M. Parry, M. D. Wang, F. M. Fernandez, V. Gilard, Analytical Chemistry, 2009, 81, 4803-4812 *Authors contributed equally to this work

  8. "Desorption Electrospray Ionization Mass Spectrometry Reveals Surface-mediated Antifungal Chemical Defense of a Tropical Seaweed", A. Lane, L. Nyadong, A. S. Galhena, E. Stout, R. Parry, M. Wang, M. Hay, F. M. Fernandez, J. Kubanek, Proceesigns of the National Academy of Sciences, 2009, 106, 7314-7319.

  9. "Influence of Cluster Size and Ion Activation on the Dissociation of Cesium Iodide Clusters", A. S. Galhena, C. M. Jones, V. H. Wysocki, International Journal of Mass Spectrometry, 2009, - Accepted

  10. "Reactive desorption electrospray ionization mass spectrometry (DESI-MS) of natural products of a marine alga.” L. Nyadong, E. G. Hohenstein, A. S. Galhena, A. Lane, J. Kubanek, C. D. Sherrill, F. M. Fernandez, Analytical and Bioanalytical Chemistry, 2009, 394, 245-254

  11. "Noncovalent Protein Tetramers and Pentamers with "n" Charges Yield Monomers with n/4 and n/5 Charges", R. L. Beardsley, C. M. Jones, A. S. Galhena, V. H. Wysocki, Analytical Chemistry, 2009, 81, 1347-1356.

  12. "Surface-Induced Dissociation of Peptides and Proteins in a Quadropole Time-of-Flight Mass Spectrometer", A. S. Galhena, S. Dagan, C. M. Jones, R. L. Beardsley, V. H. Wysocki, Analytical Chemistry, 2008, 80, 1425-1436.

  13. "Surface-Induced Dissociation Shows Potential to be More Informative Than Collision-Induced Dissociation for Structural Studies of Large Systems", V. H. Wysocki, A. S. Galhena, C. M. Jones, A. Blackwell, Journal of the American Society of Mass Spectrometry, 2008, 19, 903-913.

  14. "Symmetrical Gas-Phase Dissociation of Noncovalent Protein Complexes via Surface Collisions", C. M. Jones, R. L. Beardsley,  A. S. Galhena, S. Dagan, G. L. Cheng, V. H. Wysocki, Journal of the American Chemical Society, 2006, 128, 15044-15045.