Publications

Accepted

44. Haopeng Xiao, and Ronghu Wu*, Simultaneous quantitation of glycoprotein degradation and synthesis rates by integrating isotope labelling, chemical enrichment and multiplexed proteomics, Anal. Chem., (MS#: ac-2017-02241h), 2017.

43. Suttipong Suttapitugsakul, Haopeng Xiao, Johanna Smeekens and Ronghu Wu*,Evaluation and optimization of reduction and alkylation methods to maximize peptide identification with MS-based proteomics, Molecular BioSystems, (MB-ART-07-2017-000393), 2017.

42. Haopeng Xiao, Ju Eun Hwang and Ronghu Wu*, Mass spectrometric analysis of the N-glycoproteome in statin-treated liver cells with two lectin-independent chemical enrichment methods, Int. J. Mass Spectrom., (Invited and peer reviewed for special issue in honor of Professor Terry McMahon), 2017.

41. Moustafa R. K. Ali, Yue Wu, Yan Tang, Haopeng Xiao, Kuangcai Chen, Tiegang Han, Ning Fang*, Ronghu Wu*, Mostafa A. El-Sayed*, Targeting Cancer Cell Integrins Using Gold Nanorods in Photothermal Therapy Inhibits Migration through Affecting Cytoskeletal Proteins, Proceedings of the National Academy of Sciences, (MS#:2017-03151).

40. Mengnan Zhang, Lilya V. Matyunina, L. DeEtte Walker, Weixuan Chen, Haopeng Xiao, Benedict B. Benigno, Ronghu Wu, John F. McDonald*, Evidence for the importance of post-transcriptional regulatory changes in ovarian cancer progression and the contribution of miRNAs, Scientific Reports., (MS#:SREP-17-21574A).

2017

39. Jiangnan Zheng, Haopeng Xiao, and Ronghu Wu*, Specific Identification of Glycoproteins with the Tn Antigen in Human Cells, Angew. Chem. Int. Ed., 56:7107-7111, 2017.


 
38. Haopeng Xiao, and Ronghu Wu*, Global and site-specific analysis revealing unexpected and extensive protein S-GlcNAcylation in human cells, Analytical Chemistry, 89(6):3656-3663, 2017.
Abstract Image
 
37. Haopeng Xiao, and Ronghu Wu*, Quantitative investigation of human cell surface N-glycoprotein dynamics, Chemical Science 8 (1): 268-277, 2017.


 
36.Johanna M. Smeekens, Haopeng Xiao and Ronghu Wu*, Global Analysis of Secreted Proteins and Glycoproteins in Saccharomyces cerevisiae. Journal of Proteome Research, 16: 1039-1049, 2017.

 
35. Marian Hirushika Hettiaratchi, Catherine Chou, Nicholas Servies, Johanna M Smeekens, Albert Cheng, Camden Esancy, Ronghu Wu, Todd McDevitt, Robert Guldberg*, Laxminarayanan N Krishnan*, Competitive protein binding influences heparin-based modulation of spatial growth factor delivery for bone regeneration, Tissue Engineering 23(13-14): 683-695, 2017.

34. Moustafa RK Ali, Mohammad Aminur Rahman, Yue Wu, Tiegang Han, Xianghong Peng, Megan A Mackey, Dongsheng Wang, Hyung Ju Shin, Zhuo G Chen, Haopeng Xiao, Ronghu Wu, Yan Tang, Dong M Shin*, Mostafa A El-Sayed*, Efficacy, long-term toxicity, and mechanistic studies of gold nanorods photothermal therapy of cancer in xenograft mice, Proceedings of the National Academy of Sciences, 114(15): E3110–E3118, 2017.

2016

33. Moustafa RK Ali, Yue Wu, Tiegang Han, Xiaoling Zang, Haopeng Xiao, Yan Tang, Ronghu Wu*, Facundo Fernandez*, Mostafa, El-Sayed*, Simultaneous Time-dependent Surface Enhanced Raman Spectroscopy, Metabolomics and Proteomics Reveal Cancer Cell Death Mechanisms Associated with Au-Nanorod Photo-thermal Therapy, Journal of the American Chemical Society, 138 (47): 15434-15442, 2016.

32. Lizzette M Gómez Ramos, Johanna M Smeekens, Nicholas A Kovacs, Jessica C Bowman, Roger M Wartell, Ronghu Wu, Loren D Williams*, Yeast rRNA Expansion Segments: Folding and Function, Journal of Molecular Biology, 428(20): 4048-4059, 2016.

31. Haopeng Xiao, George X. Tang, Weixuan Chen and Ronghu Wu*, A Boronic Acid-based Enrichment for Site-specific Identification of the N-glycoproteome usingMS-based Proteomics, Book chapter in Analysis of Post-Translational Modifications and Proteolysis in Neuroscience (Editors: Jennifer Grant and Hong Li), Springer, New York, Volume 114, Page 31-41, 2016.

30. Haopeng Xiao, George X. Tang, and Ronghu Wu*, Site-specific quantification of the N-glycoproteome on the cell surface of statin-treated liver cells, Analytical Chemistry, 88(6): 3324-3332, 2016.

29. Haopeng Xiao, Johanna M. Smeekens and Ronghu Wu*, Quantification of Tunicamycin-Induced Protein Expression and N-Glycosylation Changes in Yeast, Analyst (Invited for Emerging Investigator issue, peer-reviewed), 141: 3737-3745, 2016. (themed collection Hot original research articles in Analyst 2016)
 
28. Weixuan Chen, Johanna M. Smeekens and Ronghu Wu*, Systematic study of the dynamics and half-lives of newly synthesized proteins in human cells, Chemical Science, 7(2):1393-1400, 2016.

2015

27. Weixuan Chen, Johanna M. Smeekens and Ronghu Wu*, Systematic and site-specific analysis of N-sialoglycosylated proteins on the cell surface by integrating click chemistry and MS-based proteomics, Chemical Science, 6(8):4681-4689, 2015.

 
26. Johanna M. Smeekens, Weixuan Chen, and Ronghu Wu*, Enhancing the Mass Spectrometric Identification of Membrane Proteins by Combining Chemical and Enzymatic Digestion Methods, Analytical Methods, (Invited for the Emerging Investigator themed issue and peer reviewed), 7(17):7220-7227, 2015.

 

25. Haopeng Xiao, Weixuan Chen, George X. Tang, Johanna M. Smeekens and Ronghu Wu*, Systematic Investigation of Cellular Responses and Pleiotropic Effects in Atorvastatin-treated Liver Cells by MS-based Proteomics, Journal of Proteome Research, 14(3):1600-1611, 2015.

24. Johanna M. Smeekens, Weixuan Chen and Ronghu Wu*,  Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry,  Journal of the American Society for Mass Spectrometry, (Invited for Emerging Investigators themed section and peer reviewed) DOI: 10.1007/s13361-014-1016-7, 26(4): 604-614, 2015.

2014

23. Zijun Bie, Yang Chen, Hengye Li, Ronghu Wu and Zhen Liu, Off-line hyphenation of boronate affinity monolith-based extraction with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for efficient analysis of glycoproteins/glycopeptides, Analytica Chimica Acta, 834: 1-8, 2014.

 

22. Weixuan Chen, Johanna M. Smeekens, Ronghu Wu*, A Universal Chemical Enrichment Method for Mapping the Yeast N-glycoproteome by MS, Molecular & Cellular Proteomics, 13(6):1563-1572, 2014.

 

21. Weixuan Chen, Johanna M. Smeekens, Ronghu Wu*, Comprehensive Analysis of Protein N-glycosylation Sites by Combining Chemical Deglycosylation with LC-MS, Journal of Proteome Research, 13(3):1466-1473, 2014.

 
 
Selected publications before Georgia Tech

20. Ronghu Wu, Wilhelm Haas, Noah Dephoure, Edward L. Huttlin, Bo Zhai, Mathew Sowa, Steve P. Gygi. A large-scale method to determine the absolute protein phosphorylation stoichiometry, Nature Methods, 8(8): 677-683, 2011.
 
19. Ronghu Wu, Noah Dephoure, Wilhelm Haas, Edward L. Huttlin, Bo Zhai, Mathew Sowa, Steve P. Gygi. Correct Interpretation of Comprehensive Phosphorylation Dynamics Requires Normalization by Protein Expression Changes, Molecular & Cellular Proteomics, 10(8): DOI: 10.1074/mcp.M111. 009654, 2011.
 
18. Ronghu Wu, Richard A. Marta, Jonathon K. Martens, Kris R. Eldridge, Terry B. McMahon, Experimental and Theoretical Investigation of the Proton-Bound Dimer of Lysine, Journal of the American Society for Mass Spectrometry, 22(9): 1651-1659, 2011.
 
17. Ronghu Wu, Terry B. McMahon, Structures, energetics and dynamics of gas phase ions studied by FTICR and HPMS, Mass Spectrometry Reviews, 28: 546-585, 2009.
 
16. Ronghu Wu, Terry B. McMahon, Protonation sites and conformations of peptides of Glycine (Gly1-5H+) by IRMPD spectroscopy, Journal of Physical Chemistry B, 113, 8767–8775, 2009.
 
15. Ronghu Wu, Terry B. McMahon, Investigation of Cation-π Interaction in Biological Systems, Journal of the American Chemical Society, 130(38): 12554-12555, 2008.
 
14. Ronghu Wu, Terry B. McMahon, Stabilization of Zwitterionic Structures of Amino Acids by Ammonium Ions in the Gas Phase, Journal of the American Chemical Society, 130(10): 3065-3078, 2008.

13. Ronghu Wu, Terry B. McMahon, Infrared multiple photon dissociation mechanisms of peptides of glycine (Gly1-5H+), Chemistry - A European Journal, 14(26): 7765-7770, 2008.
 
12. Ronghu Wu, Terry B. McMahon, An investigation of protonation sites and conformations of protonated amino acids by IRMPD spectroscopy, ChemPhysChem, 9: 2826-2835, 2008.
 
11. Ronghu Wu, Terry B. McMahon, IRMPD spectra of Gly·NH4+ and proton-bound betaine dimer - Evidence for zwitterionic structure, Journal of Mass Spectrometry, 43: 1641–1648, 2008.
 
10. Ronghu Wu, Terry B. McMahon, Infrared Multiple Photon Disocciation Spectroscopy as Structural Confirmation for GlyGlyGlyH+ and AlaAlaAlaH+ in the Gas Phase. Evidence for Amide Oxygen as Protonation Site, Journal of the American Chemical Society, 129(37): 11312-11313, 2007.
 
9.   Ronghu Wu, Terry B. McMahon, Infrared multiple photon dissociation spectra of proline and glycine proton-bound dimer. Evidence for zwitterionic structure, Journal of the American Chemical Society, 129(16): 4864-4865, 2007.
 
8.   Ronghu Wu, Terry B. McMahon, An Investigation of Proton Transport Tautomerism in Clusters of Protonated Nucleic Acid Bases (Cytosine, Uracil, Thymine and Adenine) and Ammonia by High Pressure Mass Spectrometry and Ab initio Calculations, Journal of the American Chemical Society, 129(3): 569-580, 2007.
 
7.   Ronghu Wu, Terry B. McMahon, Stabilization of Zwitterionic Structure of Proline by an Alkylammonium Ion in the Gas Phase, Angewandte Chemie International Edition, 46(20): 3668-3671, 2007.
 
6.   Ronghu Wu, Terry B. McMahon, An investigation of ion-molecule interactions of protonated glycine with ammonia by high pressure mass spectrometry and ab initio calculations, Canadian  Journal of Chemistry, 83(11): 1978-1993, 2005.
 
5.   Ronghu Wu, Bernhard Brutschy, Infrared depletion spectroscopy and structure of the 2-aminopyridine dimer, Journal of Physical Chemistry A, 108(45): 9715-9720, 2004.
 
4.   Ronghu Wu, Sascha Vaupel, Petr Nachtigall, Bernhard Brutschy, Structure and hydrogen bonding of different isomers of 2-aminopyridine·NH3 studied by infrared ion depletion spectroscopy, Journal of Physical Chemistry A, 108(16): 3338-3343, 2004.
 
3.   Ronghu Wu, Bernhard Brutschy, Study on the structure and intra- and intermolecular hydrogen bonding of 2-methoxyphenol·(H2O)n (n=1,2), Chemical Physics Letters, 390 (1-3): 272-278, 2004.
 
2.   Ronghu Wu, Petr Nachtigall, Bernhard Brutschy, Structure and hydrogen bonding  of 2-aminopyridine·(H2O)n (n=1,2) studied by infrared ion depletion spectroscopy, Physical Chemistry Chemical Physics, 6(3):515-521, 2004.
 
1.   Ronghu Wu, Junglee Lin, Jieli Lin, shihchang Yang, Wenbih Tzeng, Mass analyzed threshold ionization spectroscopy of N-methylaniline, N-ethylaniline, and N,N-dimethylaniline cations: Influence of N-alkyl substitution on the ionization energy and molecular vibration, The Journal of Chemical Physics, 118 (11): 4929-4937, 2003.