Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.

TitleConsensus recommendations for trabecular meshwork cell isolation, characterization and culture.
Publication TypeJournal Article
Year of Publication2018
AuthorsKeller, KE, Bhattacharya, SK, Borrás, T, Brunner, TM, Chansangpetch, S, Clark, AF, W Dismuke, M, Du, Y, Elliott, MH, C Ethier, R, Faralli, JA, Freddo, TF, Fuchshofer, R, Giovingo, M, Gong, H, Gonzalez, P, Huang, A, Johnstone, MA, Kaufman, PL, Kelley, MJ, Knepper, PA, Kopczynski, CC, Kuchtey, JG, Kuchtey, RW, Kuehn, MH, Lieberman, RL, Lin, SC, Liton, P, Liu, Y, Lütjen-Drecoll, E, Mao, W, Masis-Solano, M, McDonnell, F, McDowell, CM, Overby, DR, Pattabiraman, PP, Raghunathan, VK, P Rao, V, Rhee, DJ, Chowdhury, URoy, Russell, P, Samples, JR, Schwartz, D, Stubbs, EB, Tamm, ER, Tan, JC, Toris, CB, Torrejon, KY, Vranka, JA, Wirtz, MK, Yorio, T, Zhang, J, Zode, GS, Fautsch, MP, Peters, DM, Acott, TS, W Stamer, D
JournalExp Eye Res
Volume171
Pagination164-173
Date Published2018 06
ISSN1096-0007
KeywordsAge Factors, Animals, Biomarkers, Cell Culture Techniques, Cell Separation, Consensus, Fetus, Guidelines as Topic, Humans, Tissue and Organ Harvesting, Tissue Donors, Tissue Preservation, Trabecular Meshwork
Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

1
DOI10.1016/j.exer.2018.03.001
Alternate JournalExp. Eye Res.
PubMed ID29526795
PubMed Central IDPMC6042513
Grant ListI01 RX001163 / RX / RRD VA / United States
R21 EY028671 / EY / NEI NIH HHS / United States
R01 EY026009 / EY / NEI NIH HHS / United States
P30 EY016665 / EY / NEI NIH HHS / United States
R01 EY017006 / EY / NEI NIH HHS / United States
R01 EY025643 / EY / NEI NIH HHS / United States
R01 EY026885 / EY / NEI NIH HHS / United States
R01 EY021205 / EY / NEI NIH HHS / United States
K99 EY022077 / EY / NEI NIH HHS / United States
R01 EY023242 / EY / NEI NIH HHS / United States
P30 EY000331 / EY / NEI NIH HHS / United States
R01 EY019643 / EY / NEI NIH HHS / United States
K08 EY024674 / EY / NEI NIH HHS / United States
R01 EY011906 / EY / NEI NIH HHS / United States
R01 EY026177 / EY / NEI NIH HHS / United States
R01 GM100909 / GM / NIGMS NIH HHS / United States
R01 EY027733 / EY / NEI NIH HHS / United States
R01 EY026220 / EY / NEI NIH HHS / United States
R21 EY027079 / EY / NEI NIH HHS / United States
R00 EY022077 / EY / NEI NIH HHS / United States