|Title||Nonenzymatic ligation of DNA with a reversible step and a final linkage that can be used in PCR.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Engelhart, AE, Cafferty, BJ, C Okafor, D, Chen, MC, Williams, LDean, Lynn, DG, Hud, NV|
|Date Published||2012 May 29|
|Keywords||Combinatorial Chemistry Techniques, DNA, DNA Probes, DNA-Directed DNA Polymerase, Morpholines, Polymerase Chain Reaction|
Nonenzymatic DNA ligation chemistries containing a reversible step allow thermodynamic control of product formation, but they are not necessarily compatible with polymerase enzymes. We report a ligation system that uses commercially available reagents, includes a reversible step, and results in a linkage that can function as a template for PCR amplification with accurate sequence transfer.