DNA-templated Ag nanocluster formation.

TitleDNA-templated Ag nanocluster formation.
Publication TypeJournal Article
Year of Publication2004
AuthorsPetty, JT, Zheng, J, Hud, NV, Dickson, RM
JournalJ Am Chem Soc
Date Published2004 Apr 28
KeywordsCircular Dichroism, DNA, Kinetics, Magnetic Resonance Spectroscopy, Nucleic Acid Conformation, Oligonucleotides, Silver, Spectrometry, Fluorescence, Spectrometry, Mass, Electrospray Ionization, Templates, Genetic

The high affinity of Ag+ for DNA bases has enabled creation of short oligonucleotide-encapsulated Ag nanoclusters without formation of large nanoparticles. Time-dependent formation of cluster sizes ranging from Ag1 to Ag4/oligonucleotide were observed with strong, characteristic electronic transitions between 400 and 600 nm. The slow nanocluster formation kinetics enables observation of specific aqueous nanocluster absorptions that evolve over a period of 12 h. Induced circular dichroism bands confirm that the nanoclusters are associated with the chiral ss-DNA template. Fluorescence, absorption, mass, and NMR spectra all indicate that multiple species are present, but that their creation is both nucleotide- and time-dependent.

Alternate JournalJ. Am. Chem. Soc.
PubMed ID15099104
Grant List1R01GM068731 / GM / NIGMS NIH HHS / United States