Publications
M. T. Morgan, D. Bourassa, S. Harankhedkar, A. M. McCallum, S. A. Zlatic, J. S. Calvo, G. Meloni, V. Faundez, C. J. Fahrni, “Ratiometric two-photon microscopy reveals attomolar copper buffering in normal and Menkes mutant cells”, Proc. Natl. Acad. Sci. U.S.A. 2019, 116, 12167-12172. [pubmed] [link]
C. Hartwig, S. A. Zlatic, M. Wallin, A. Vrailas-Mortimer, C. J. Fahrni, V. Faundez, “Trafficking mechanisms of P-type ATPase copper transporters”, Curr. Opin. Cell Biol. 2019, 59, 24-33. [pubmed] [link]
C.M. Elitt, C.J. Fahrni, P.A. Rosenberg, “Zinc homeostasis and zinc signaling in white matter development and injury”, Neurosci. Lett. 2019, 707, 134247. [pubmed] [link]
J. Yu and C.J. Fahrni, “In Situ Imaging of Zinc with Synthetic Fluorescent Probes”, in Zinc Signaling, p. 365-388, Ed. T. Fukada, T. Kambe, Springer, Nature Singapore 2019. [link]
F. Saeedifard, M. T. Morgan, J. Bacsa, C. J. Fahrni,“Preorganized PSP Ligands Yield Monomeric Cu(I) Complexes with Subzeptomolar Cu(I) Dissociation Constants“, Inorg. Chem. 2019, 58, 13631-13638. [pubmed] [link]
D. M. Heuberger, S. Harankhedkar, M. T. Morgan, P. Wolint, M. Calcagni, B. Lai, C. J. Fahrni, J. Buschmann, “High-affinity Cu(I) chelator PSP-2 as potential anti-angiogenic agent”, Sci. Rep. 2019, 9, 1-10. [pubmed] [link]
M.T. Morgan, B. Yang, S. Harankhedkar, A. Nabatilan, D. Bourassa, A.M. McCallum, F. Sun, R. Wu, C.R. Forest, C.J. Fahrni, "Stabilization of Aliphatic Phosphines by Auxiliary Phosphine Sulfides Offers Zeptomolar Affinity and Unprecedented Selectivity for Probing Biological Cu(I)", Angew. Chem. Int. Ed. Engl. 2018, 57, 9711-9715. [pubmed] [link]
D. Bourassa, C.M. Elitt, A.M. McCallum, S. Sumalekshmy, R.L. McRae; M.T. Morgan, N. Siegel, J.W. Perry, P.A. Rosenberg, C.J. Fahrni, "Chromis-1, a Ratiometric Fluorescent Probe Optimized for Two-Photon Microscopy Reveals Dynamic Changes in Labile Zn(II) in Differentiating Oligodendrocytes", ACS Sensors 2018, 3, 458-467. [pubmed] [link]
M.T. Morgan, L.A.H. Nguyen, H.L. Hancock, C.J. Fahrni, "Glutathione limits aquacopper(I) to sub-femtomolar concentrations through cooperative assembly of a tetranuclear cluster", J. Biol. Chem. 2017, 292, 21558-21567. [pubmed] [link]
C.J. Fahrni, D. Bourassa, R. Dikdan, "Probing Biological Trace Metals with Fluorescent Indicators", Metals in the Brain: Measurement and Imaging, ed. Anthony R. White, Springer, 2017. [link]
D. Bourassa, S.C. Gleber, S. Vogt, C.H. Shin, C.J. Fahrni, "MicroXRF tomographic visualization of zinc and iron in the zebrafish embryo at the onset of the hatching period", Metallomics 2016, 8, 1122-1130. [pubmed] [link]
M.T. Morgan, A.M. McCallum, C.J. Fahrni, "Rational design of a water-soluble, lipid-compatible fluorescent probe for Cu (I) with sub-part-per-trillion sensitivity", Chem. Sci. 2016, 7, 1468-1473. [pubmed] [link]
Y.C. Chen, A.E. Jablonski, I. Issaeva, D. Bourassa, J.C. Hsiang, C.J. Fahrni, R.M. Dickson, "Optically modulated photoswitchable fluorescent proteins yield improved biological imaging sensitivity", J. Am. Chem. Soc. 2015, 40, 12764-12767. [pubmed] [link]
F. Friscourt, C.J. Fahrni, G.J. Boons, "Fluorogenic strain-promoted alkyne-diazo cycloadditions", Chem. Eur. J. 2015, 21, 13996-14001. [pubmed] [link]
M.T. Morgan, S. Sumalekshmy, M. Sarwar, H. Beck, S. Crooke, C.J. Fahrni, "Probing ternary complex equilibria of crown ether ligands by time-resolved fluorescence spectroscopy", J. Phys. Chem. B 2014, 118, 14196-14202. [pubmed] [link]
D. Bourassa, S.-C. Gleber, S. Vogt, H. Yi, F. Will, H. Richter, C.H. Shin, C.J. Fahrni, "3D Imaging of Transition Metals in the Zebrafish Embryo by X-ray Fluorescence Microtomography”, Metallomics 2014, 6, 1648-1655. [pubmed] [link]
P. Bagchi, M.T. Morgan, J. Bacsa, C.J. Fahrni, “Robust affinity standards for Cu(I) biochemistry”, J. Am. Chem. Soc. 2013, 135, 18549-59. [pubmed] [link]
McRae, R.; Lai, B.; Fahrni, C. J., "Subcellular redistribution and mitotic inheritance of transition metals in proliferating mouse fibroblast cells.", Metallomics 2013, 5, 52-61. [pubmed] [link]
Fahrni, C. J., "Synthetic fluorescent probes for monovalent copper.", Curr. Opin. Chem. Biol. 2013, 17, 656-62 [pubmed] [link]
Morgan, M. T.; Bagchi, P.; Fahrni, C. J., "High-contrast fluorescence sensing of aqueous Cu(I) with triarylpyrazoline probes: dissecting the roles of ligand donor strength and excited state proton transfer.", Dalton Trans. 2013, 42, 3240-3248. [pubmed] [link]
Nekongo, E. E.; Bagchi, P.; Fahrni, C. J.; Popik, V. V., "9-Aryl-9-xanthenols: a convenient platform for the design of fluorimetric and colorimetric pH indicators.", Org. Biomol. Chem. 2012, 10, 9214-9218. [pubmed] [link]
Jablonski, A.; Hsiang, J. C.; Bagchi, P.; Hull, N.; Richards, C. I.; Fahrni, C. J.; Dickson, R. M., "Signal discrimination between fluorescent proteins in live cells by long-wavelength optical modulation", J. Phys. Chem. Lett. 2012, 3, 3585-3591. [pubmed] [link]
Friscourt, F.; Fahrni, C. J.; Boons, G.-J., "A fluorogenic probe for the catalyst-free detection of azide-tagged molecules.", J. Am. Chem. Soc. 2012, 134, 18809-18815. [pubmed] [link]
Sumalekshmy, S.; Fahrni, C. J., "Metal-ion-responsive fluorescent probes for two-photon excitation microscopy", Chem. Mat. 2011, 823-830. [link]
Morgan, M. T.; Bagchi, P.; Fahrni, C. J., "Designed to dissolve: Suppression of colloidal aggregation of Cu(I)-selective fluorescent probes in aqueous buffer and in-gel detection of a metallochaperone.", J. Am. Chem. Soc. 2011, 133, 15906-15909. [pubmed] [link]
Chaudhry, A. F.; Mandal, S.; Hardcastle, K. I.; Fahrni, C. J., "High-contrast Cu(I)-selective fluorescent probes based on synergistic electronic and conformational switching", Chem. Sci. 2011, 2, 1016-1024. [pubmed] [link]
Verma, M.; Chaudhry, A. F.; Morgan, M. T.; Fahrni, C. J., "Electronically tuned 1,3,5-triarylpyrazolines as Cu(I)-selective fluorescent probes", Org. Biomol. Chem. 2010, 8, 363-370. [pubmed] [link]
McRae, R.; Lai, B.; Fahrni, C. J., "Copper redistribution in Atox1-deficient mouse fibroblast cells", J. Biol. Inorg. Chem. 2010, 15, 99-105. [pubmed] [link]
Chaudhry, A. F.; Verma, M.; Morgan, M. T.; Henary, M. M.; Siegel, N.; Hales, J. M.; Perry, J. W.; Fahrni, C. J., "Kinetically Controlled Photoinduced Electron Transfer Switching in Cu(I)-Responsive Fluorescent Probes", J. Am. Chem. Soc. 2010, 132, 737-747. [pubmed] [link]
Verma, M.; Chaudhry, A. F.; Fahrni, C. J., "Predicting the photoinduced electron transfer thermodynamics in polyfluorinated 1,3,5-triarylpyrazolines based on multiple linear free energy relationships", Org. Biomol. Chem. 2009, 7, 1536-1546. [pubmed] [link]
McRae, R.; Bagchi, P.; Sumalekshmy, S.; Fahrni, C. J., "In situ imaging of metals in cells and tissues", Chem. Rev. 2009, 109, 4780-4827. [pubmed] [link]
Fahrni, C. J., "Fluorescent probes and labels for cellular imaging", Chimia 2009, 63, 714-720. [link]
Solntsev, K. M.; McGrier, P. L.; Fahrni, C. J.; Tolbert, L. M.; Bunz, U. H. F., "Anomalous photophysics of bis(hydroxystyryl)benzenes: A twist on the para/meta dichotomy", Org. Lett. 2008, 10, 2429-2432. [pubmed] [link]
McRae, R. L.; Phillips, R. L.; Kim, I. B.; Bunz, U. H. F.; Fahrni, C. J., "Molecular recognition based on low-affinity polyvalent interactions: Selective binding of a carboxylated polymer to fibronectin fibrils of live fibroblast cells", J. Am. Chem. Soc. 2008, 130, 7851-7853. [pubmed] [link]
Cody, J.; Mandal, S.; Yang, L. C.; Fahrni, C. J., "Differential tuning of the electron transfer parameters in 1,3,5-triarylpyrazolines: A rational design approach for optimizing the contrast ratio of fluorescent probes", J. Am. Chem. Soc. 2008, 130, 13023-13032. [pubmed] [link]
Wu, Y. G.; Lawson, P. V.; Henary, M. M.; Schmidt, K.; Bredas, J. L.; Fahrni, C. J., "Excited state intramolecular proton transfer in 2-(2 '-arylsulfonamidophenyl)benzimidazole derivatives: Insights into the origin of donor substituent-induced emission energy shifts", J. Phys. Chem. A 2007, 111, 4584-4595. [pubmed] [link]
Sumalekshmy, S.; Henary, M. M.; Siegel, N.; Lawson, P. V.; Wu, Y.; Schmidt, K.; Bredas, J. L.; Perry, J. W.; Fahrni, C. J., "Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness", J. Am. Chem. Soc. 2007, 129, 11888-11889. [pubmed] [link]
Henary, M. M.; Wu, Y. G.; Cody, J.; Sumalekshmy, S.; Li, J.; Mandal, S.; Fahrni, C. J., "Excited-state intramolecular proton transfer in 2-(2 '-arylsulfonamidophenyl)benzimidazole derivatives: The effect of donor and acceptor substituents", J. Org. Chem. 2007, 72, 4784-4797. [pubmed] [link]
Fahrni, C. J., "Biological applications of X-ray fluorescence microscopy: exploring the subcellular topography and speciation of transition metals", Curr. Opin. Chem. Biol. 2007, 11, 121-127. [pubmed] [link]
McRae, R.; Lai, B.; Vogt, S.; Fahrni, C. J., "Correlative microXRF and optical immunofluorescence microscopy of adherent cells labeled with ultrasmall gold particles", J. Struct. Biol. 2006, 155, 22-29. [pubmed] [link]
Yang, L.; McRae, R.; Henary, M. M.; Patel, R.; Lai, B.; Vogt, S.; Fahrni, C. J., "Imaging of the intracellular topography of copper with a fluorescent sensor and by synchrotron x-ray fluorescence microscopy.", Proc. Natl. Acad. Sci. USA 2005, 102, 11179-11184. [pubmed] [link]
Zhou, Z.; Fahrni, C. J., "A fluorogenic probe for the copper(I)-catalyzed azide-alkyne ligation reaction: Modulation of the fluorescence emission via (3)(n,pi*)-(1)(pi,pi*) inversion", J. Am. Chem. Soc. 2004, 126, 8862-8863. [pubmed] [link]
Samia, A. C. S.; Cody, J.; Fahrni, C. J.; Burda, C., "The effect of ligand constraints on the metal-to-ligand charge-tranfer relaxation dynamics of copper(I)-phenanthroline complexes: A comparative study by femtosecond time-resolved spectroscopy", J. Phys. Chem. B 2004, 108, 563-569. [link]
Henary, M. M.; Wu, Y. G.; Fahrni, C. J., "Zinc(II)-selective ratiometric fluorescent sensors based on inhibition of excited-state intramolecular proton transfer", Chem. Eur. J. 2004, 10, 3015-3025. [pubmed] [link]
Cody, J.; Fahrni, C. J., "Fluorescence sensing based on cation-induced conformational switching: copper-selective modulation of the photoinduced intramolecular charge transfer of a donor-acceptor biphenyl fluorophore", Tetrahedron 2004, 60, 11099-11107. [link]
Fahrni, C. J.; Yang, L. C.; VanDerveer, D. G., "Tuning the photoinduced electron-transfer thermodynamics in 1,3,5-triaryl-2-pyrazoline fluorophores: X-ray structures, photophysical characterization, computational analysis, and in vivo evaluation", J. Am. Chem. Soc. 2003, 125, 3799-3812. [pubmed] [link]
Cody, J.; Dennisson, J.; Gilmore, J.; VanDerveer, D. G.; Henary, M. M.; Gabrielli, A.; Sherrill, C. D.; Zhang, Y. Y.; Pan, C. P.; Burda, C.; Fahrni, C. J., "X-ray structures, photophysical characterization, and computational analysis of geometrically constrained copper(I)-phenanthroline complexes", Inorg. Chem. 2003, 42, 4918-4929. [pubmed] [link]
Henary, M. M.; Fahrni, C. J., "Excited state intramolecular proton transfer and metal ion complexation of 2-(2'-hydroxyphenyl)benzazoles in aqueous solution", J. Phys. Chem. A 2002, 106, 5210-5220. [link]
Fahrni, C. J.; Henary, M. M.; VanDerveer, D. G., "Excited-state intramolecular proton transfer in 2-(2'-tosylaminophenyl)benzimidazole", J. Phys. Chem. A 2002, 106, 7655-7663. [link]
Nasir, M. S.; Fahrni, C. J.; Suhy, D. A.; Kolodsick, K. J.; Singer, C. P.; O'Halloran, T. V., "The chemical cell biology of zinc: structure and intracellular fluorescence of a zinc-quinolinesulfonamide complex", J. Biol. Inorg. Chem. 1999, 4, 775-783. [pubmed] [link]
Fahrni, C. J.; Simon, K. D.; Suhy, D. A.; Nasir, M. S.; Dwivedi, R.; O'Halloran, T. V., "Investigation of intracellular zinc storage and transport with zinc specific fluorescence probes", J. Inorg. Biochem. 1999, 74, 125-125.
Fahrni, C. J.; O'Halloran, T. V., "Aqueous coordination chemistry of quinoline-based fluorescence probes for the biological chemistry of zinc", J. Am. Chem. Soc. 1999, 121, 11448-11458. [link]
Fahrni, C. J.; Pfaltz, A.; Neuburger, M.; Zehnder, M., "Structure and properties of transition-metal complexes with chiral C-2-symmetric binucleating ligands", Helv. Chim. Acta 1998, 81, 507-524. [link]
Fahrni, C. J.; Pfaltz, A., "Synthesis of chiral C2-symmetric binucleating ligands", Helv. Chim. Acta 1998, 81, 491-506. [link]
Fahrni, C. J., "Allylic oxidation catalyzed by chiral dinuclear copper complexes", Tetrahedron 1998, 54, 5465-5470. [link]
Pufahl, R. A.; Singer, C. P.; Peariso, K. L.; Lin, S. J.; Schmidt, P. J.; Fahrni, C. J.; Culotta, V. C.; Penner-Hahn, J. E.; O'Halloran, T. V., "Metal ion chaperone function of the soluble Cu(I) receptor Atx1.", Science 1997, 278, 853-856. [pubmed] [link]
